Serena Williams withdrew from the WTA clay-court tournament that begins Monday in Rome due to a left Achilles tendon injury sustained in her US Open semi-final defeat, the tour announced Saturday.
Williams, chasing her record-tying 24th Grand Slam single crown, lost to Victoria Azarenka in three sets on Thursday on the Arthur Ashe Stadium hardcourt.
The former world number one, a four-time Rome champion, joins 2019 US Open winner Bianca Andreescu and two-time Wimbledon winner Petra Kvitova is skipping the tuneup for the French Open, which begins on September 27.
Williams took a medical timeout in her New York loss to have the Achilles injury treated.
Top-ranked Ashleigh Barty withdrew from Rome earlier in the week, the Australian star saying she woul shut down her season due to coronavirus concerns and travel restrictions.